We, Japan SLC, Inc. produce the laboratory animals, according to the standard operating procedures under the stable environment. Also, we maintain and control a quality and characteristics of the animals produced by microbial and genetic monitoring.
Production Style
For production of the uniform laboratory animal which maintained characteristics, maintenance and production of the strain is performed by strict production system based on the production control standard.
Outbred
- Maintenance and production by rotation breeding scheme
Inbred
- Maintained colony: Strain maintenance and production of seed animals by the brother-sister mating
- Multiplication colony: Multiplication of seed animals used to Production colony and Hybrid (F1) production colony by the brother-sister mating in the maintained colony
- Production colony: Animal production by random mating in multiplication colony
Hybrid
- Hybrid (F1) production colony by mating between females and males of different inbred strain
Disease Models
- Production by preserving genetic characters and keeping their characteristics
Breeding Environment
Animals are bred and cared in the following environment;
| Room temperature |
Mouse・Rat・Hamster・Mongolian gerbil ： 23～25°C Nude mouse ： 24～26°C Guinea pig ： 21～25°C Rabbit ： 20～24°C |
| Humidity | 45～65% Nude mouse only ： 50～70% |
| Ventilation frequency | All fresh air 10～15times/hour |
| Differential pressure | 50～200Pa (5～20mmH2O) |
| Illuminance | 150～300Lux (80cm above the floor) |
| Lighting cycle | Light (7:00～20:00), Dark (20:00～8:00) Hamster only ： Light (7:00～21:00), Dark (21:00～7:00) |
| Noise | ≤ 60 decibel (dB) |
| Odor | Ammonia concentration ≤ 20ppm |
| Air | Sterilized by 3-stage filters; front, middle, final (HEPA) |
| Aeration | Pan-type air conditioning system is used as flow system |
Breeding Environmental Monitoring
Examination of drinking water and bedding, and performance tests of autoclave and ethylene oxide gas sterilizers are periodically performed.
Animal Behavioral Monitoring
Production efficiency of each production colony is monitored and recorded in weekly report.
Breeding technical person carefully observes animal and detects early abnormal animal.
Feeds
Animals are given following diet;
Autoclaved diets (Nosan Corporation, PMI Inc.)
Irradiated diets (PMI Inc., Nosan Corporation, Funabashi Farm Co.,Ltd.)
Microbiological Monitoring
- １) Only specified strains
Genetic Monitoring
Mouse and Rat Inbred Strains
Test items
- Biochemical, immunogenetic and molecular-genetic markers (Microsatellite and SNP).
Test animals
- Animals from each foundation stock colony and multiplication colony.
Test frequencies
- At least once a year for each test item in each strain.
Physiological Parameter Tests
Body weight measurement
Organ weight measurement
- Test items：brain・heart・lung・liver・spleen・kidney・adrenal・testis・ovary・thymus・pituitary gland
Hematological tests
- Test items：RBC・Ht・Hb・WBC・PLT・MCV・MCH・MCHC
Blood biochemical tests
- Test items：AST・ALT・ALP・TG・ALB・TP・A/G・GLU・PL・T-BIL・BUN・T-CHO・Ca・IP・CRE・Na・K・Cl