In accordance with the standard production system of SLC, the experimental animals of SLC are produced with stringent microbiological and genetic control under certain environmental standards, and then monitored periodically to assure that each product maintain its characteristic.
The report of microbiological tests is issued once every month.
The report of genetic tests is issued once every year.


With the aim of producing ewell characterized and uniform experimental animalse,we undertake maintenance and production of well defined strains of experimental animals under the standard production system including stringent microbiological and genetic control.
iPjClosed Colonies
  • Maintained and produced by the cyclic mating system
iQjInbred
  • Foundation stock coloniesFThe eseed stocke of each strain is maintained and produced by brother-to-sister mating.
  • Multiplication colonies: Animals from stock colonies are multiplied by brother- to-sister mating to produce production colony animals and F1 production animals.
  • Production colonies:FThese colonies consist of animals from multiplication colonies and they are multiplied by non-brother-to-sister mating to produce laboratory animals to be used for experiments.
iRjHybrid
  • Stock animals from each inbred multiplication colony are cross-bred to produce F1 animals


We breed animals in barriers-system breeding rooms
under the following environmental condition.
temperature mouseEratEhamsterE23`25
mongolian gerbilEEEE24`26
Guinea pigEEEEEEEEE23`25
rabbitEEEEEEEEEEEEE20`24
humidity 45`65%
Ventilation
frequency
All fresh air 10`15times/hour
Static pressure difference 50`200Pai5`20mmH2Oj
Lighting 150`300Lux i80cm above the floor)
Light( 8:00`20:00)Adark(20:00`8:00)
Air Sterilized by 3-stage filters,
front,middle,final(HEPA)
Aeration Pan-type air conditioner diffuser
blowout style



Autoclaved solid pellets from Nihon Nosan
KogyoK.K.and PMI and irradiated solid pellets
from PMI and Funabashi Farm are used.

Microbiological MonitoringiSPF animalj
ijSPF animal only
Items Method(1) Frequency(2) Mouse Rat Hamster
Mongolian
gerbil
Guineapig
Rabbit
Pseudomonas aeruginosaijCulture 2times/month
Citrobacter rodentium Culture 2times/month ~ ~ ~ ~ ~
Salmonella spp. Culture 2times/month
Salmonella typhimurium Serology test 2times/month
Pasteurella pneumotropica Culture 2times/month ~
Pasteurella multocida Culture 2times/month ~ ~ ~ ~ ~
Bordetella bronchiseptica (3) Culture E
Serology test
2times/month ~
Streptococcus pneumoniae Culture 2times/month ~ ~ ~
Streptococcus zooepidemicus Culture 2times/month ~ ~ ~ ~ ~
Staphylococcus aureus (4) Culture 2times/month ~ ~ ~ ~ ~
Corynebacterium kutscheri Culture E
Serology test
2times/month ~ ~
Clostridium piliforme Serology test 2times/month
Helicobacter hepaticus PCR 4times/year(mouse)
2times/year(rat)
~ ~ ~
Helicobacter bilis PCR 4times/year(mouse)
2times/year(rat)
~ ~ ~
CAR bacilus Serology test 1times/year ~ ~ ~ ~
Mycoplasma pulmonis (5) CultureE
Serology test
2times/month
Mouse hepatitis virus Serology test 2times/month ~ ~ ~ ~ ~
Sialodacryoadenitis virus Serology test 2times/month ~ ~ ~ ~ ~
Sendai virus Serology test 2times/month
Hantavirus Serology test3times/year ~ ~ ~ ~
Pneumonia virus of mice Serology test 1times/year ~ ~ ~
Mouse adenovirus Serology test 1times/year ~ ~ ~ ~
Lymphocytic choriomeningitis virus Serology test 3times/year ~ ~ ~ ~
Ectromelia virus Serology test 3times/year ~ ~ ~ ~ ~
Murine norovirus PCR 1time/year ~ ~ ~ ~ ~
Pinworm Microscopic test 2times/month ~ ~
Intestinal protozoa Microscopic test 2times/month ~ ~
Eimeria caviae Microscopic test 2times/month ~ ~ ~ ~ ~
Eimeria spp. Microscopic test 2times/month ~ ~ ~ ~ ~
Ectoparasits Microscopic test 2times/month ~
Psoroptes cuniculi Microscopic test 2times/month ~ ~ ~ ~ ~
Pneumocystis carinii(4) Microscopic testEPCR 2times/year ~ ~ ~ ~ ~
Dermatophytes Culture 2times/year ~ ~ ~ ~
(1) The microbiological monitoring is executed based on the pathogen diagnostics of the microbiological monitoring manual of SLC. When abnormality is seen while monitoring it, we will execute the inspection that seems to be necessary it.
(2)
EAbnormal animalEEEEEEEThe one that abnormality was seen in each breeding room.
AttentionF.The age,several animals,and the examination frequency change according to the scale of production.
(3)The animal species other than the guinea pig and the rabbit execute only the culture test.
(4)This item becomes only BALB/cSlc- nu/nu and KSN/Slc.
(5)The mouse and the rat execute the culture test and serum reaction(ELISA method ,Once ever three month),and other animals execute only the culture test.



The following tests are periodically performed:
Quality tests for drinking water,setting microbe tests as an indicator of microbiological cleanliness
of the environmental air,and function tests of the high pressure and ethylene gas sterilizers to guarantee aseptic conditions of the instruments and apparatuses to be used.


Production efficiency of each production colony is monitored and recorded in weekly report. Husbandry technicians at work try to observe animals carefully to detect any abnormality in behavior at an early stage.



Mouse Inbred StrainsERat Inbred strains
Genetic monitoring is carried out at least once a year for inbred mouse and rat strains.
The test items are as follows
1.Biochemical markers
EDetection of isozyme and protein variants using electrophoretic mobility or enzymatic activity.
2.Immunogenetic markers
EDetection of the various H2K and H2D (the major histocompatibility complex in the mouse) alleles using cytotoxicity test.
RT1.A(the major histocompatibility complex in the rat) alleles are tested by Devision of Genetics,ICLAS Monitoring Center.
3.Microsatellite markers
EDetection of polymorphisms of microsatellite DNA using PCR method.
4.Mutant genes in spontaneous mutants
EConfirmation of mutant genes(Faslpr and Faslgld) using PCR or PCR-RELP method.
Sample number for each test item:
3 males and 3 females/strain
All tests are in accordance with the Genetic monitoring SOP of Japan SLC,Inc.


  • Body weight measurement
    • Organ weight measurement
      • Test organsFbrainEheartElungEliverEspleenEkidneyEadrenalEtestisEovaryEthymus
    • Tests for pregnant animalsiratErabbitj
      • Test itemsFUteral interiorAskeletalAand organ tests
    • Hematological tests
      • Blood is sampled from the abdominal aorta(by heart puncture in guines pigs and rabbits)under ether anesthesia.
      • Test itemsFRBCEHtEHbEWBCEBPEMCVEMCHEMCHC
    • Blood biochemical tests
      • Blood is sampled from the abdominal aorta(by heart puncture in guinea pigs and rabbits) under ether anesthesia.
      • Test itemsFTPEALBEA/GEASTEALTEALPEGLUET.CHOETGEPLET.BILEBUNECREEIPECaE NaEKECl
    • All these data are reported in the SLC data book.

Copyright(C) 2005 by Japan SLC, Inc. All Rights Reserved.